Steps for Acclimation of Your New Aquatic Life:
1. Turn off all aquarium lights to reduce stress on aquatic life.
2. Thoroughly wash your hands to remove perfumes, lotions, etc.
3. Float the sealed bag in your aquarium for at least 5 minutes, (Figure 1) but no longer than one hour to allow for temperature acclimation. The water in the bag should be the same temperature as that of your tank prior to proceeding to the next step. (If, for whatever reason, the bag has burst or the water is extremely foul, do not temperature acclimate; proceed directly to step 9.)
4. After floating the bag for 5 minutes, carefully cut it open as close to the top as possible and fold the top edge of the bag down one inch to create an air pocket within the lip of the bag. This air pocket will allow the bag to float on the surface of the water. Or, if possible, secure the bag to the aquarium side with an algae or lettuce clip.
5. Slowly add 1/8 to1/4 cup of aquarium water to the bag. (Figure 2) It may be easiest to use 1/8 cup for small bags and 1/4 cup for large bags.
6. Repeat step 5 every five minutes until the bag is full.
7. Once the bag is full, remove the bag from the aquarium and discard half of the water in the bag into a bucket, being careful not to harm your aquatic life.
8. Re-float the bag in the aquarium, (Figure 3) and slowly add 1/4 to 1/2 cup of aquarium water to the bag every five minutes until the bag is full (see steps 4-6). This will further acclimate the new aquatic life to your system and will eliminate most of the water originally in the bag.
9. Once the bag is full, remove it from the aquarium and slowly pour off as much water as possible into a bucket without harming your aquatic life (see step 7).
10. Grasp the bottom corner of the bag and lower it into the aquarium.
11. Lift the bottom corner of the bag and allow your fish to swim out of the bag. (Figure 4) If you have an invertebrate, submerse the bag and carefully remove the invertebrate (remember to only handle corals by the base).
12. Any remaining water in the bag should be poured into a bucket or sink, not the tank.